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Journal: Journal of genetics and genomics = Yi chuan xue bao
Article Title: Serological targeted analysis of an ITIH4 peptide isoform: A preterm birth biomarker and its associated SNP implications
doi: 10.1016/j.jgg.2015.06.001
Figure Lengend Snippet: A: Representative LCMS ion chromatogram. The red vertical line indicates that the ITIH4 “L” peptide precursor ion with the matching MS/MS spectrum was found eluting at 16.1 min. B: An MS/MS spectrum of precursory ion m/z 1006.26 (2+) that matched to the ITIH4 “L” peptide with sequence LLGLPGPPDVPDHAAYHPF. C: ITIH4 “L” isoform peptide chromatogram derived from preterm birth cases (gray) and healthy pregnancy controls (black). The peaks in the chromatogram were formed by the elution of “L” isoform peptides at the HPLC 16th time point, and the ionic intensities of ITIH4 “L” isoform peptide were normalized with the stable isotope labeled spiked-in ITIH4 “L*” isoform peptide. The normalized “L” isoform peptide abundances, plotted with the standard deviations, were higher in healthy pregnancy controls than in preterm birth cases. D: Scatter plot analysis of each subject’s normalized serum abundance as a function of the baby gestational age at the time of sample collection. Violet red represents preterm birth cases; sea green represents full term pregnancy controls. For either preterm birth or full term birth, bars of 25th, 50th and 75th percentiles represent and compare the overall trend of biomarker scoring as a function of the sample classification. E: ITIH4 “L” isoform peptide abundance in term delivery pregnancy sera of different gestational ages. Comparative analysis of ITIH4 “L” peptide serum abundances between early (< 35 weeks) and late (≥ 35 weeks) GA revealed no statistically significant difference between the two groups (Student’s t test, P = 0.747). F: ROC analysis of the ITIH4 “L” isoform serum peptide as a preterm birth biomarker. 500 testing data sets, generated by bootstrapping, from the normalized abundance data were used to derive estimates of standard errors and confidence intervals for our ROC analysis. The plotted ROC curve is the vertical average of the 500 bootstrapping runs, and the box and whisker plots show the vertical spread around the average. The red star denotes the cut point with the optimal sensitivity and specificity of the assay. Summary of the cut point performance of sensitivity specificity, positive predictive value, ROC AUC and their 95% confidence intervals are listed in the right table.
Article Snippet: To test these hypotheses, we firstly applied quantitative proteomic methods to identification of the ITIH4 “L” isoform peptide in normal pregnancy sera. shows a
Techniques: Tandem Mass Spectroscopy, Sequencing, Derivative Assay, Labeling, Biomarker Discovery, Generated, Whisker Assay